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dc.contributor.authorEichler, Paulopt_BR
dc.contributor.authorBastiani, Daniela dept_BR
dc.contributor.authorSantos, Fernando Almeidapt_BR
dc.contributor.authorAyub, Marco Antônio Záchiapt_BR
dc.date.accessioned2023-08-23T03:30:23Zpt_BR
dc.date.issued2020pt_BR
dc.identifier.issn0001-3765pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/263906pt_BR
dc.description.abstractWe evaluated the conditions to produce lipase in solid-state cultivation using a recently isolated strain of Aspergillus brasiliensis 157f in bioreactors of different confi gurations: static fl at-bed, plugged-fl ow bed with forced water-saturated aeration, and pilot-scale rotating drum bioreactor, using malt bagasse as substrate. Lipase production was optimized applying experimental design analysis, which showed optima parameters defi ned as pH 7.7, addition of 11.3 % of soybean oil to the medium, and culture temperature of 32.7 o C, in static fl at-bed. The highest enzyme activity (9.8 U.g-1 substrate) was obtained in the plugged-fl ow bed with forced water-saturated aeration. The fermented culture medium was lyophilized to create a solid enzymatic preparation (SEP), which was used to test the possibility of using this cheap biocatalyst in bioreactors to mediate esterifi cation and transesterifi cation reactions. SEP presented lipase activities of 7.35 U.g-1 substrate, indicating the possibility of further enhancing aspects of the use of such biocatalyst.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofAnais da Academia Brasileira de Ciências. Rio de Janeiro. Vol. 92, suppl. 2 (2020), e20180856, 15 p.pt_BR
dc.rightsOpen Accessen
dc.subjectAspergillus brasiliensisen
dc.subjectAspergilluspt_BR
dc.subjectBrewery’s spent malten
dc.subjectLipasept_BR
dc.subjectMaltept_BR
dc.subjectLipase productionen
dc.subjectSolid-state cultivationen
dc.subjectTransesterificaçãopt_BR
dc.subjectTransesterification reactionen
dc.titleLipase production by Aspergillus brasiliensis in solid-state cultivation of malt bagasse in different bioreactors configurationspt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb001174150pt_BR
dc.type.originNacionalpt_BR


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