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dc.contributor.authorKuhl, Leonardo Palmapt_BR
dc.contributor.authorMarostica, Paulo José Cauduropt_BR
dc.contributor.authorMacedo, Alexandre Josépt_BR
dc.contributor.authorKuhl, Gabrielpt_BR
dc.contributor.authorSiebert, Marinapt_BR
dc.contributor.authorManica, Denisept_BR
dc.contributor.authorSekine, Leopt_BR
dc.contributor.authorSchweiger, Claudiapt_BR
dc.date.accessioned2023-06-17T03:37:48Zpt_BR
dc.date.issued2023pt_BR
dc.identifier.issn1808-8694pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/259125pt_BR
dc.description.abstractObjectives To evaluate the bacterial microbiome found in tracheostomy cannulas of a group of children diagnosed with glossoptosis secondary to Robin Sequence (RS), and its clinical implications. Methods Pediatric patients were enrolled in the study at the time of the cannula change in the hospital. During this procedure, the removed cannula was collected and stored for amplicon sequencing of 16s rRNA. DNA extraction was performed using DNeasy PowerBiofilm Kit (QIAGEN® ‒ Cat nº 24000-50) while sequencing was performed with the S5 (Ion S5™ System, Thermo Fisher Scientific), following Brazilian Microbiome Project (BMP) protocol. Results All 12 patients included in the study were using tracheostomy uncuffed cannulas of the same brand, had tracheostomy performed for over 1-year and had used the removed cannula for approximately 3-months. Most abundant genera found were Aggregatibacter, Pseudomonas, Haemophilus, Neisseria, Staphylococcus, Fusobacterium, Moraxella, Streptococcus, Alloiococcus, and Capnocytophaga. Individual microbiome of each individual was highly variable, not correlating to any particular clinical characteristic. Conclusion The microbiome of tracheostomy cannulas is highly variable, even among patients with similar clinical characteristics, making it challenging to determine a standard for normality.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofBrazilian journal of otorhinolaryngology. São Paulo. Vol. 89, n. 2 (Mar./Apr. 2023), p. 254-263pt_BR
dc.rightsOpen Accessen
dc.subjectChilden
dc.subjectMicrobiotapt_BR
dc.subjectTracheostomyen
dc.subjectFenômenos microbiológicospt_BR
dc.subjectMetagenomicsen
dc.subjectTraqueostomiapt_BR
dc.subject16S rRNAen
dc.subjectCriançapt_BR
dc.subjectMetagenômicapt_BR
dc.subjectRNA ribossômico 16Spt_BR
dc.titleHigh microbiome variability in pediatric tracheostomy cannulas in patients with similar clinical characteristicspt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb001166053pt_BR
dc.type.originNacionalpt_BR


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