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dc.contributor.authorAndrade, Miguel de Souzapt_BR
dc.contributor.authorCampos, Fabrício Souzapt_BR
dc.contributor.authorFranco, Ana Claudiapt_BR
dc.contributor.authorRoehe, Paulo Michelpt_BR
dc.contributor.authorAbreu, Felipe Vieira Santos dept_BR
dc.date.accessioned2023-02-10T04:54:50Zpt_BR
dc.date.issued2022pt_BR
dc.identifier.issn0074-0276pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/254540pt_BR
dc.description.abstractBACKGROUND In Brazil, the yellow fever virus (YFV) is maintained in a sylvatic cycle involving wild mosquitoes and nonhuman primates (NHPs). The virus is endemic to the Amazon region; however, waves of epidemic expansion reaching other Brazilian states sporadically occur, eventually causing spillovers to humans. OBJECTIVES To report a surveillance effort that led to the first confirmation of YFV in NHPs in the state of Minas Gerais (MG), Southeast region, in 2021. METHODS A surveillance network was created, encompassing the technology of smartphone applications and coordinated actions of several research institutions and health services to monitor and investigate NHP epizootics. FINDINGS When alerts were spread through the network, samples from NHPs were collected and YFV infection confirmed by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and genome sequencing at an interval of only 10 days. Near-complete genomes were generated using the Nanopore MinION sequencer. Phylogenetic analysis indicated that viral genomes were related to the South American genotype I, clustering with a genome detected in the Amazon region (state of Pará) in 2017, named YFVPA/MG sub-lineage. Fast YFV confirmation potentialised vaccination campaigns. MAIN CONCLUSIONS A new YFV introduction was detected in MG 6 years after the beginning of the major outbreak reported in the state (2015-2018). The YFV strain was not related to the sub-lineages previously reported in MG. No human cases have been reported, suggesting the importance of coordinated surveillance of NHPs using available technologies and supporting laboratories to ensure a quick response and implementation of contingency measures to avoid YFV spillover to humans.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoporpt_BR
dc.relation.ispartofMemórias do Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Vol. 117 (2022), e220127, 9 p.pt_BR
dc.rightsOpen Accessen
dc.subjectYellow fever virusen
dc.subjectVírus da febre amarelapt_BR
dc.subjectServiços de vigilância epidemiológicapt_BR
dc.subjectArbovirusen
dc.subjectFlavivirusen
dc.subjectSequenciamento por nanoporospt_BR
dc.subjectNon-human primateen
dc.subjectFilogeniapt_BR
dc.subjectEpizooticen
dc.subjectSmartphoneen
dc.subjectMinIONen
dc.titleFast surveillance response reveals the introduction of a new yellow fever virus sub-lineage in 2021, in Minas Gerais, Brazilpt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb001156949pt_BR
dc.type.originNacionalpt_BR


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