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dc.contributor.authorOz, Mehmet Tufanpt_BR
dc.contributor.authorAltpeter, Angelikapt_BR
dc.contributor.authorKaran, Ratnapt_BR
dc.contributor.authorMerotto Junior, Aldopt_BR
dc.contributor.authorAltpeter, Fredypt_BR
dc.date.accessioned2023-01-02T05:09:11Zpt_BR
dc.date.issued2021pt_BR
dc.identifier.issn1664-462xpt_BR
dc.identifier.urihttp://hdl.handle.net/10183/253335pt_BR
dc.description.abstractSugarcane is the source of 80% of the sugar and 26% of the bioethanol produced globally. However, its complex, highly polyploid genome (2n = 100 – 120) impedes crop improvement. Here, we report efficient and reproducible gene targeting (GT) in sugarcane, enabling precise co-editing of multiple alleles via template-mediated and homology-directed repair (HDR) of DNA double strand breaks induced by the programmable nuclease CRISPR/Cas9. The evaluation of 146 independently transformed plants from five independent experiments revealed a targeted nucleotide replacement that resulted in both targeted amino acid substitutions W574L and S653I in the acetolactate synthase (ALS) in 11 lines in addition to single, targeted amino acid substitutions W574L or S653I in 25 or 18 lines, respectively. Co-editing of up to three ALS copies/alleles that confer herbicide tolerance was confirmed by Sanger sequencing of cloned long polymerase chain reaction (PCR) amplicons. This work will enable crop improvement by conversion of inferior alleles to superior alleles through targeted nucleotide substitutions.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofFrontiers in Genome Editing. Lausanne. Vol. 3 (July 2021), 673566, 12 p.pt_BR
dc.rightsOpen Accessen
dc.subjectResistência à pesticidapt_BR
dc.subjectHomologous recombinationen
dc.subjectCana de açúcarpt_BR
dc.subjectCRISPR/Cas9en
dc.subjectSugarcane (Saccharum hybrid complex)en
dc.subjectResistência genéticapt_BR
dc.subjectRecombinaçãopt_BR
dc.subjectGenome editingen
dc.subjectGene targetingen
dc.subjectRecombinação de homólogospt_BR
dc.subjectHomology directed repairen
dc.titleCRISPR/Cas9-mediated multi-allelic gene targeting in sugarcane confers herbicide tolerancept_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb001153389pt_BR
dc.type.originEstrangeiropt_BR


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