Hepatitis C virus genotypes in Southern Brazil
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Abstract
The prevalence of hepatitis C virus (HCV) genotypes in Southern Brazil was studied in the plasma of 100 HCV-RNA-positive patients attended in Porto Alegre, South of Brazil. Reverse transcriptionpolymerase chain reaction (RT-PCR) products from the 5' noncoding region were double digested with Rsal-Haeiii and BstNl-Hinfi and analyzed by restriction fragment length polymorphism (RFLP). Three genotypes (1, 2 and 3) were demonstrable, the most prevalent being HCV type I (55 of 100 patients, 55%), fo ...
The prevalence of hepatitis C virus (HCV) genotypes in Southern Brazil was studied in the plasma of 100 HCV-RNA-positive patients attended in Porto Alegre, South of Brazil. Reverse transcriptionpolymerase chain reaction (RT-PCR) products from the 5' noncoding region were double digested with Rsal-Haeiii and BstNl-Hinfi and analyzed by restriction fragment length polymorphism (RFLP). Three genotypes (1, 2 and 3) were demonstrable, the most prevalent being HCV type I (55 of 100 patients, 55%), followed by HCV type 3 (37 of 100 patients, 37%) and HCVtype 2 (8 oflOO patients, 8%). There was an unusual high prevalence of genotype 3, in contrast to the majority of published data from the Southeast region. ...
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Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas. Ribeirão Preto. Vol. 29, n. 12 (dec. 1996), p. 1629-1632
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