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dc.contributor.advisorBattastini, Ana Maria Oliveirapt_BR
dc.contributor.authorJandrey, Elisa Helena Fariaspt_BR
dc.date.accessioned2022-05-13T04:52:38Zpt_BR
dc.date.issued2015pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/238582pt_BR
dc.description.abstractBackground/Aim: Glioblastoma multiforme is the deadliest tumor of central-nervous-system, consisting mainly of tumor and immune cells. T-regulatory cells can modulate to a pro-tumor microenvironment. Microvesicles (MVs), among them exosomes, are responsible of cell-to-cell interaction and are involved in the modulation of tumor-microenvironment. We describe the isolation and characterization of MVs in an in vitro glioma model. Materials and Methods: We performed 4 tests to determine best conditions to isolation of MVs (size and protein content). After determine the best condition, MVs were isolated through ultracentrifugation, characterized by size (ZetaSizer/Nanosight) and protein content. Results: Room temperature thaw and additional centrifugation (test 3) was the test that has the best results for size and protein content. BCA is the better technique to quantify protein concentration of MVs. Nanosight analysis demonstrates minor size variations. MVs show a size increase after five days at 4°C. The treatment with MVs decreases viability of C6 cells in vitro. Conclusion: Successful isolation/characterization of MVs.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoporpt_BR
dc.rightsOpen Accessen
dc.subjectFarmáciapt_BR
dc.titleIsolamento e caracterização de microvesículas derivadas de gliomaspt_BR
dc.typeTrabalho de conclusão de graduaçãopt_BR
dc.identifier.nrb001020400pt_BR
dc.degree.grantorUniversidade Federal do Rio Grande do Sulpt_BR
dc.degree.departmentFaculdade de Farmáciapt_BR
dc.degree.localPorto Alegre, BR-RSpt_BR
dc.degree.date2015pt_BR
dc.degree.graduationFarmáciapt_BR
dc.degree.levelgraduaçãopt_BR


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