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dc.contributor.authorBreyer, Gabriela Merkerpt_BR
dc.contributor.authorDias, Maria Eduardapt_BR
dc.contributor.authorHenker, Luan Cleberpt_BR
dc.contributor.authorLorenzett, Marina Paulapt_BR
dc.contributor.authorBaumbach, Leticia Ferreirapt_BR
dc.contributor.authorCanal, Cláudio Wageckpt_BR
dc.contributor.authorPavarini, Saulo Petinattipt_BR
dc.contributor.authorSiqueira, Franciele Mabonipt_BR
dc.date.accessioned2021-10-27T04:27:02Zpt_BR
dc.date.issued2021pt_BR
dc.identifier.issn1678-0345pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/231375pt_BR
dc.description.abstractBackground: Pregnancy losses are a major concern in livestock industry due to their economic impact on producers. Campylobacter fetus subspecies fetus (Cff) and C. fetus subspecies venerealis (Cfv) are directly related to reproductive failures in ruminants. Cff colonizes the gastrointestinal tract of a wide range of hosts leading to abortion, while Cfv is restricted to genital tract being generally associated to infertility in bovine. Considering the great economic losses related to campylobacteriosis in cattle and ovine herds, this study aims to investigate the occurrence of C. fetus, considering Cff and Cfv subspecies, in bovine and ovine spontaneously aborted fetuses in state of Rio Grande do Sul, Brazil. Materials, Methods & Results: In this study, samples of abomasal fluid collected from 30 spontaneously aborted bovine (n = 18) and ovine (n = 12) fetuses were investigated for the detection of Campylobacter fetus throughout conventional PCR. Positive fetuses for C. fetus presence were further analyzed by molecular assays for Cff and Cfv detection, in order to determine subspecies identification. When available, samples of the main organs of the thoracic and abdominal cavities, as well as the brain, skeletal muscle, eyelid, skin, and placenta were collected for further histopathological analyses and bacterial culture, aiming to assess the presence of infection lesions and pathogens in those sites, respectively. Additionally, RT-qPCR assays were also performed for the detection of ruminant pestivirus, in order to detect bovine viral diarrhea cases. Throughout the present methodology, C. fetus was detected in the abomasal fluid samples of 2 bovine fetuses, being both identified as Cfv subspecies by PCR. Histopathological analyses demonstrated that macroscopic and microscopic changes found in the Cfv-positive animals were not either specific or directly related to Campylobacter infections. Moreover, no significant bacterial growth was observed in microbiological culture from the collected tissues, and both fetuses were negative for ruminant pestivirus. Differently, there was no detection of C. fetus in any of the analyzed ovine fetuses. Discussion: Considering that abortion diagnosis rates reported in cattle and sheep industry are highly variable among the published studies, and that abortion diagnoses are commonly inconclusive due to difficulties in sampling methodology and inadequate identification of the pathogen involved, it is important to investigate the etiological causes of abortion the herds for better understanding the causes of pregnancy issues and monitoring their occurrence. In addition, the absence of pathognomonic lesions in the tissues investigated in the histopathological analyses observed in this study strongly suggests that well-known etiological agents commonly associated to abortion, such as Leptospira spp., Toxoplasma spp., Chlamydia spp. and Neospora caninum, are unlikely to be the cause of infection of the analyzed fetuses. Taking this into account, the presence of C. fetus in the abomasal fluid samples from two bovine fetuses demonstrated in the present study suggests the possible association of Cfv not only with infertility, but also with cases of bovine abortion, highlighting the importance of investigating unusual causal agents of abortions in sheep and cattle. Overall, an adequate diagnosis is essential for establishing better prevention strategies to avoid the circulation of abortion-related infectious agents in the herds.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofActa scientiae veterinariae. Porto Alegre, RS. Vol. 49 (2021), Pub. 1834, 7 p.pt_BR
dc.rightsOpen Accessen
dc.subjectInfecções por Campylobacterpt_BR
dc.subjectCampylobacteriosisen
dc.subjectVenereal diseaseen
dc.subjectInfecções por pestiviruspt_BR
dc.subjectReproductive diseaseen
dc.subjectAborto espontâneopt_BR
dc.subjectBovinospt_BR
dc.subjectAbortionen
dc.subjectOvinospt_BR
dc.subjectMolecular diagnosisen
dc.subjectRio Grande do Sulpt_BR
dc.titleCampylobacter fetus in abomasal fluid from spontaneously aborted bovine and ovine fetusespt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb001131977pt_BR
dc.type.originNacionalpt_BR


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