Mostrar el registro sencillo del ítem

dc.contributor.authorLamers, Marcelo Lazzaronpt_BR
dc.contributor.authorAlmeida, Maíra Estanislau Soarespt_BR
dc.contributor.authorVicente-Manzanares, Miguelpt_BR
dc.contributor.authorHorwitz, Alan F.pt_BR
dc.contributor.authorSantos, Marinilce Fagundes dospt_BR
dc.date.accessioned2021-08-06T04:41:02Zpt_BR
dc.date.issued2011pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/225249pt_BR
dc.description.abstractDeficient wound healing in diabetic patients is very frequent, but the cellular and molecular causes are poorly defined. In this study, we evaluate the hypothesis that high glucose concentrations inhibit cell migration. Using CHO.K1 cells, NIH-3T3 fibroblasts, mouse embryonic fibroblasts and primary skin fibroblasts from control and diabetic rats cultured in 5 mM Dglucose (low glucose, LG), 25 mM D-glucose (high glucose, HG) or 25 mM L-glucose medium (osmotic control - OC), we analyzed the migration speed, protrusion stability, cell polarity, adhesion maturation and the activity of the small Rho GTPase Rac1. We also analyzed the effects of reactive oxygen species by incubating cells with the antioxidant N-AcetylCysteine (NAC). We observed that HG conditions inhibited cell migration when compared to LG or OC. This inhibition resulted from impaired cell polarity, protrusion destabilization and inhibition of adhesion maturation. Conversely, Rac1 activity, which promotes protrusion and blocks adhesion maturation, was increased in HG conditions, thus providing a mechanistic basis for the HG phenotype. Most of the HG effects were partially or completely rescued by treatment with NAC. These findings demonstrate that HG impairs cell migration due to an increase in oxidative stress that causes polarity loss, deficient adhesion and protrusion. These alterations arise, in large part, from increased Rac1 activity and may contribute to the poor wound healing observed in diabetic patients.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofPLoS ONE [recurso eletrônico]. São Francisco, CA. Vol. 6, no. 8 (Aug. 2011), p. e22865pt_BR
dc.rightsOpen Accessen
dc.subjectPatologiapt_BR
dc.subjectDiabetespt_BR
dc.subjectCicatrizaçãopt_BR
dc.titleHigh glucose-mediated oxidative stress impairs cell migrationpt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb000794810pt_BR
dc.type.originEstrangeiropt_BR


Ficheros en el ítem

Thumbnail
   

Este ítem está licenciado en la Creative Commons License

Mostrar el registro sencillo del ítem