Induction of selective liver hypothermia prevents significant ischemia/reperfusion injury in Wistar rats after 24 hours
dc.contributor.author | Grezzana Filho, Tomáz de Jesus Maria | pt_BR |
dc.contributor.author | Longo, Larisse | pt_BR |
dc.contributor.author | Santos, Jorge Luiz dos | pt_BR |
dc.contributor.author | Gabiatti, Gémerson | pt_BR |
dc.contributor.author | Corso, Carlos Otavio | pt_BR |
dc.contributor.author | Bofill, Carlos Medeiros | pt_BR |
dc.contributor.author | Santos, Emanuel Burck dos | pt_BR |
dc.contributor.author | Cerski, Carlos Thadeu Schmidt | pt_BR |
dc.contributor.author | Chedid, Márcio Fernandes | pt_BR |
dc.contributor.author | Corso, Carlos Otavio | pt_BR |
dc.date.accessioned | 2021-08-05T04:29:48Z | pt_BR |
dc.date.issued | 2020 | pt_BR |
dc.identifier.issn | 0102-8650 | pt_BR |
dc.identifier.uri | http://hdl.handle.net/10183/225143 | pt_BR |
dc.description.abstract | Purpose: To investigate the effects of induction of selective liver hypothermia in a rodent model. Methods: Seven male Wistar rats were subjected to 90 minutes of partial 70% liver ischemia and topic liver 26°C hypothermia (H group). Other seven male Wistar rats were subjected to 90 minutes of partial 70% normothermic liver ischemia (N group). Five additional rats underwent a midline incision and section of liver ligaments under normothermic conditions and without any liver ischemia (sham group). All animals were sacrificed 24-h after reperfusion, and livers were sampled for analyses. Pathology sections were scored for sinusoidal congestion, ballooning, hepatocelllular necrosis and the presence of neutrophilic infiltrates. Results: At the end of the experiment, liver tissue expressions of TNF-ɑ, IL-1β, iNOS and TNF-ɑ/IL-10 ratio were significantly reduced in the H group compared to N group, whereas IL-10 and eNOS were significantly increased in H group. Histopathological injury scores revealed a significant decrease in ischemia/reperfusion (I/R) injuries in H group. Conclusion: Selective liver hypothermia prevented I/R injury by inhibiting the release of inflammatory cytokines, preserves microcirculation, prevents hepatocellular necrosis and leukocyte infiltration, allowing maintenance of the liver architecture. | en |
dc.format.mimetype | application/pdf | pt_BR |
dc.language.iso | eng | pt_BR |
dc.relation.ispartof | Acta cirúrgica brasileira. São Paulo. Vol. 35, n. 2 (2020) e202000205, 13 p. | pt_BR |
dc.rights | Open Access | en |
dc.subject | Cytokines | en |
dc.subject | Citocinas | pt_BR |
dc.subject | Hypothermia | en |
dc.subject | Hipotermia | pt_BR |
dc.subject | Inflammation | en |
dc.subject | Fígado | pt_BR |
dc.subject | Inflamação | pt_BR |
dc.subject | Liver | en |
dc.subject | Rats | en |
dc.subject | Modelos animais | pt_BR |
dc.title | Induction of selective liver hypothermia prevents significant ischemia/reperfusion injury in Wistar rats after 24 hours | pt_BR |
dc.type | Artigo de periódico | pt_BR |
dc.identifier.nrb | 001129351 | pt_BR |
dc.type.origin | Nacional | pt_BR |
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