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dc.contributor.authorRomariz, Simone Amaro Alvespt_BR
dc.contributor.authorPaiva, Daisyléa de Souzapt_BR
dc.contributor.authorValente, Maria Fernandapt_BR
dc.contributor.authorBarnabé, Gabriela Filosopt_BR
dc.contributor.authorFrussa-Filho, Robertopt_BR
dc.contributor.authorSilva, Regina Cláudia Barbosa dapt_BR
dc.contributor.authorCalcagnotto, Maria Elisapt_BR
dc.contributor.authorLongo, Beatriz Monteiropt_BR
dc.date.accessioned2021-07-21T04:24:38Zpt_BR
dc.date.issued2014pt_BR
dc.identifier.issn1471-2202pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/224306pt_BR
dc.description.abstractBackground: The GABAergic system plays an important role in modulating levels of anxiety. When transplanted into the brain, precursor cells from the medial ganglionic eminence (MGE) have the ability to differentiate into GABAergic interneurons and modify the inhibitory tone in the host brain. Currently, two methods have been reported for obtaining MGE precursor cells for transplantation: fresh and neurosphere dissociated cells. Here, we investigated the effects generated by transplantation of the two types of cell preparations on anxiety behavior in rats. Results: We transplanted freshly dissociated or neurosphere dissociated cells into the neonate brain of male rats on postnatal (PN) day 2–3. At early adulthood (PN 62–63), transplanted animals were tested in the Elevated Plus Maze (EPM). To verify the differentiation and migration pattern of the transplanted cells in vitro and in vivo, we performed immunohistochemistry for GFP and several interneuron-specific markers: neuropeptide Y (NPY), parvalbumin (PV) and calretinin (CR). Cells from both types of preparations expressed these interneuronal markers. However, an anxiolytic effect on behavior in the EPM was observed in animals that received the MGE-derived freshly dissociated cells but not in those that received the neurosphere dissociated cells. Conclusion: Our results suggest a long-lasting anxiolytic effect of transplanted freshly dissociated cells that reinforces the inhibitory function of the GABAergic neuronal circuitry in the hippocampus related to anxiety-like behavior in rats.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofBMC neuroscience. London. Vol. 15 (2 Aug. 2014), 94 [8] p.pt_BR
dc.rightsOpen Accessen
dc.subjectAnxietyen
dc.subjectAnsiedadept_BR
dc.subjectMedial ganglionic eminenceen
dc.subjectÁcido gama-aminobutíricopt_BR
dc.subjectNeuronal precursor cellsen
dc.subjectNeurôniospt_BR
dc.subjectHipocampopt_BR
dc.subjectTransplantationen
dc.subjectTransplantept_BR
dc.titleLong-lasting anxiolytic effect of neural precursor cells freshly prepared but not neurosphere-derived cell transplantation in newborn ratspt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb000980613pt_BR
dc.type.originEstrangeiropt_BR


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