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dc.contributor.authorAlvarado Rincón, Joao Alveiropt_BR
dc.contributor.authorMion, Brunapt_BR
dc.contributor.authorVelasco Acosta, Diego Andrespt_BR
dc.contributor.authorGasperin, Bernardo Garzierapt_BR
dc.contributor.authorRovani, Monique Tomazelept_BR
dc.contributor.authorPegoraro, Lígia Margareth Cantarellipt_BR
dc.contributor.authorCorrêa, Márcio Nunespt_BR
dc.contributor.authorSchneider, Augustopt_BR
dc.date.accessioned2021-07-21T04:24:11Zpt_BR
dc.date.issued2019pt_BR
dc.identifier.issn1806-9614pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/224276pt_BR
dc.description.abstractThe aim of this study was to evaluate the long term effects of recombinant bovine somatotropin (rbST) on follicle population and ovulatory follicle development in non-lactating dairy cows. Twenty-one Jersey cows were allocated in rbST (n=11) or control (n=10) groups. On day -60, cows in rbST group received 500 mg of somatotropin (s.c. Lactotropin, Elanco). On day 0, control and rbST cows received an intravaginal progesterone-releasing device (1.9 g, CIDR, Zoetis) and GnRH (100 mg, IM, Factrel, Zoetis). On day 8, cows received PGF2α (25 mg, IM, Lutalyse, Zoetis) and the CIDR was removed. Twelve hours after device removal (D8), serum, follicular fluid and granulosa cells samples were collected. Serum and follicular concentration of estradiol (E2) and progesterone (P4) were analyzed. Total RNA was extracted from granulosa cells to measure gene expression of LHCGR, STAR, HSD-3B1, CYP11A1, CYP19A1, CYP17A1, IGFR and PAPPA by real time PCR. Ultrasonography was performed on days -60, -53, -46, -14, -7, 0 and 8 for antral follicle count. Results were analyzed by repeated measures ANOVA and t-test. There was no effect of rbST treatment on the number of follicles during the 60 days period, as well as no effect on serum and follicular fluid E2 and follicular fluid P4 at the moment of follicle aspiration. There was a reduction in PAPPA (P = 0.006), CYP11A1 (P = 0.04) and CYP19A1 (P = 0.002) mRNA levels in granulosa cells of the pre ovulatory follicle of rbST treated cows. In conclusion, a single dose of rbST did not have long term effects on antral follicle population, serum and follicular E2/P4 concentrations in non-lactating dairy cows. Despite that, rbST injection decreased granulosa cell expression of genes related to steroidogenesis in the pre-ovulatory follicle.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofAnimal reproduction. Belo Horizonte. Vol. 16, n. 4 (2019), p. 914-922pt_BR
dc.rightsOpen Accessen
dc.subjectHormônio do crescimentopt_BR
dc.subjectFollicular populationen
dc.subjectGene expressionen
dc.subjectFolículo ovarianopt_BR
dc.subjectSteroidogenesisen
dc.subjectExpressão gênicapt_BR
dc.subjectCélulas da granulosapt_BR
dc.subjectVacas leiteiraspt_BR
dc.titleEffect of recombinant bovine somatotropin (rbST) treatment on follicular population and development in non-lactating dairy cowspt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb001127483pt_BR
dc.type.originNacionalpt_BR


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