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dc.contributor.authorAraújo, Ivana Maria Zaccara Cunhapt_BR
dc.contributor.authorJardine, Alexander Pompermayerpt_BR
dc.contributor.authorMestieri, Leticia Boldrinpt_BR
dc.contributor.authorQuintana, Ramiro Martinspt_BR
dc.contributor.authorJesus, Luciano Henrique dept_BR
dc.contributor.authorMoreira, Maria Stella Nunes Araújopt_BR
dc.contributor.authorGrecca, Fabiana Soarespt_BR
dc.contributor.authorMartins, Manoela Dominguespt_BR
dc.contributor.authorKopper, Patrícia Maria Polipt_BR
dc.date.accessioned2021-05-25T04:25:19Zpt_BR
dc.date.issued2019pt_BR
dc.identifier.issn1806-8324pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/221464pt_BR
dc.description.abstractThis study aimed to evaluate the role of photobiomodulation (PBM) in apexification and apexogenesis of necrotic rat molars with an open apex. Rat molars were exposed to the oral environment for 3 weeks. Canals were rinsed with 2.5% NaOCl and 17% EDTA, filled with antibiotic paste and sealed. After 7 days, canals were rinsed and divided into six groups (n=6): mineral trioxide aggregate (MTA); blood clot (BC); human dental pulp stem cells (hDPSC); MTA+PBM; BC+PBM; and hDPSC+PBM. In hDPSC groups, a 1% agarose gel scaffold was used. Two groups were not exposed: healthy tooth+PBM (n = 6), healthy tooth (n = 3); and one was exposed throughout the experiment: necrotic tooth (n = 3). In PBM groups, irradiation was performed with aluminum gallium indium phosphide (InGaAlP) diode laser for 30 days within 24-h intervals. After that, the specimens were processed for histological and immunohistochemical analyses. Necrotic tooth showed greater neutrophil infiltrate (p < 0.05). Necrotic tooth, healthy tooth, and healthy tooth+PBM groups showed absence of a thin layer of fibrous condensation in the periapical area. All the other groups stimulated the formation of a thicker layer of fibers (p < 0.05). All groups formed more mineralized tissue than necrotic tooth (p < 0.05). PBM associated with MTA, BC, or hDPSC formed more mineralized tissue (p < 0.05). MTA+PBM induced apexification (p < 0.05). Rabbit polyclonal anti-bone sialoprotein (BSP) antibody confirmed the histological findings of mineralized tissue formation, and hDPSC groups exhibited higher percentage of BSP-positive cells. It can be concluded that PBM improved apexification and favored apexogenesis in necrotic rat molars with an open apex.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofBrazilian oral research. São Paulo. Vol. 33 (2019), e084, p. 1-13pt_BR
dc.rightsOpen Accessen
dc.subjectLow-Level Light Therapyen
dc.subjectTerapia com luz de baixa intensidadept_BR
dc.subjectPolpa dentáriapt_BR
dc.subjectDental Pulpen
dc.subjectStem Cellsen
dc.subjectCélulas-troncopt_BR
dc.subjectDental Pulp Necrosisen
dc.subjectNecrose da polpa dentáriapt_BR
dc.subjectApexificaçãopt_BR
dc.subjectApexificationen
dc.subjectDente molarpt_BR
dc.subjectMolaren
dc.subjectRatospt_BR
dc.subjectRatsen
dc.titleInfluence of photobiomodulation therapy on root development of rat molars with open apex and pulp necrosispt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb001107078pt_BR
dc.type.originNacionalpt_BR


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