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dc.contributor.authorMann, Michele Bertonipt_BR
dc.contributor.authorPrichula, Janirapt_BR
dc.contributor.authorCastro, Ícaro Maia Santos dept_BR
dc.contributor.authorSevero, Juliana Mellopt_BR
dc.contributor.authorVasconcellos, Michelle Abadie dept_BR
dc.contributor.authorLima, Thayná Mendes de Freitaspt_BR
dc.contributor.authorCaorsi, Valentina Zaffaronipt_BR
dc.contributor.authorMartins, Márcio Borgespt_BR
dc.contributor.authorFrazzon, Jeversonpt_BR
dc.contributor.authorFrazzon, Ana Paula Guedespt_BR
dc.date.accessioned2021-04-06T04:19:37Zpt_BR
dc.date.issued2021pt_BR
dc.identifier.issn2076-2607pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/219469pt_BR
dc.description.abstractMelanophryniscus admirabilis (admirable red-belly toad) is a microendemic and critically endangered species found exclusively along 700 m of the Forqueta River, in a fragment of the Atlantic Forest of southern Brazil. One of the greatest concerns regarding the conservation of this species is the extensive use of pesticides in areas surrounding their natural habitat. In recent years, the adaptation and persistence of animal species in human-impacted environments have been associated with microbiota. Therefore, the present study aimed to characterize the oral bacterial community of wild M. admirabilis and to address the question of how this community might contribute to this toad’s adaptation in the anthropogenic environment as well as its general metabolic capabilities. A total of 11 oral samples collected from wild M. admirabilis were characterized and analyzed via high-throughput sequencing. Fragments of the 16S rRNA variable region 4 (V4) were amplified, and sequencing was conducted using an Ion Personal Genome Machine (PGM) System with 316 chips. A total of 181,350 sequences were obtained, resulting in 16 phyla, 34 classes, 39 orders, and 77 families. Proteobacteria dominated (53%) the oral microbiota of toads, followed by Firmicutes (18%), Bacteroidetes (17%), and Actinobacteria (5%). No significant differences in microbial community profile from among the samples were reported, which suggests that the low dietary diversity observed in this population may directly influence the bacterial composition. Inferences of microbiome function were performed using PICRUSt2 software. Important pathways (e.g., xenobiotic degradation pathways for pesticides and aromatic phenolic compounds) were detected, which suggests that the bacterial communities may serve important roles in M. admirabilis health and survival in the anthropogenic environment. Overall, our results have important implications for the conservation and management of this microendemic and critically endangered species.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofMicroorganisms. Basel. Vol. 9, no. 2 (Feb. 2021), 220, 11 p.pt_BR
dc.rightsOpen Accessen
dc.subjectHigh-throughput sequencingen
dc.subjectBufonidaept_BR
dc.subjectAmphibianen
dc.subjectSequenciamento de nucleotídeos em larga escalapt_BR
dc.subjectBacteriaen
dc.subjectBocapt_BR
dc.subjectMicrobiotapt_BR
dc.subjectXenobioticen
dc.subjectAnthropogenic actionen
dc.subjectAnálise de sequência de RNApt_BR
dc.titleThe oral bacterial community in Melanophryniscus admirabilis (admirable red-belly toads) : implications for conservationpt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb001123111pt_BR
dc.type.originEstrangeiropt_BR


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