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dc.contributor.authorPranke, Patricia Helena Lucaspt_BR
dc.contributor.authorHendrikx, Janpt_BR
dc.contributor.authorAlespeite, Gabrielpt_BR
dc.contributor.authorNardi, Nance Beyerpt_BR
dc.contributor.authorRubinstein, Pablopt_BR
dc.contributor.authorVisser, Janpt_BR
dc.date.accessioned2010-04-24T04:15:41Zpt_BR
dc.date.issued2006pt_BR
dc.identifier.issn0100-879Xpt_BR
dc.identifier.urihttp://hdl.handle.net/10183/21197pt_BR
dc.description.abstractThe total number of CD34+ cells is the most relevant clinical parameter when selecting human umbilical cord blood (HUCB) for transplantation. The objective of the present study was to compare the two most commonly used CD34+ cell quantification methods (ISHAGE protocol and ProCount™ - BD) and analyze the CD34+ bright cells whose 7-amino actinomycin D (7AAD) analysis suggests are apoptotic or dead cells. Twenty-six HUCB samples obtained at the Placental Blood Program of New York Blood Center were evaluated. The absolute numbers of CD34+ cells evaluated by the ISHAGE (with exclusion of 7AAD+ cells) and ProCount™ (with exclusion of CD34+ bright cells) were determined. Using the ISHAGE protocol we found 35.6 ± 19.4 CD34+ cells/μL and with the ProCount™ method we found 36.6 ± 23.2 CD34+ cells/μL. With the ProCount™ method, CD34+ bright cell counts were 9.3 ± 8.2 cells/μL. CD34+ bright and regular cells were individually analyzed by the ISHAGE protocol. Only about 1.8% of the bright CD34+ cells are alive, whereas a small part (19.0%) is undergoing apoptosis and most of them (79.2%) are dead cells. Our study showed that the two methods produced similar results and that 7AAD is important to exclude CD34 bright cells. These results will be of value to assist in the correct counting of CD34+ cells and to choose the best HUCB unit for transplantation, i.e., the unit with the greatest number of potentially viable stem cells for the reconstitution of bone marrow. This increases the likelihood of success of the transplant and, therefore, the survival of the patient.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofBrazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas. Ribeirão Preto, SP. Vol. 39, n. 7 (Jul. 2006), p. 901-906pt_BR
dc.rightsOpen Accessen
dc.subjectHematopoietic stem cellen
dc.subjectCélulas-tronco hematopoéticaspt_BR
dc.subjectCordão umbilicalpt_BR
dc.subjectHuman umbilical cord blooden
dc.subjectCD34+en
dc.subjectISHAGEen
dc.subjectProCounten
dc.titleComparative quantification of umbilical cord blood CD34+ and CD34+ bright cells using the procount tm-bd and ishage protocolspt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb000544257pt_BR
dc.type.originNacionalpt_BR


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