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dc.contributor.authorAchaval-Elena, Matildept_BR
dc.contributor.authorPenha, Marco Aurélio Pereirapt_BR
dc.contributor.authorSwarowsky, Alessandrapt_BR
dc.contributor.authorSoster, Paula Rigon da Luzpt_BR
dc.contributor.authorXavier, Leder Lealpt_BR
dc.contributor.authorViola, Giordano Gubertpt_BR
dc.contributor.authorZancan, Denise Mariapt_BR
dc.date.accessioned2010-04-24T04:15:39Zpt_BR
dc.date.issued2005pt_BR
dc.identifier.issn0100-879Xpt_BR
dc.identifier.urihttp://hdl.handle.net/10183/21190pt_BR
dc.description.abstractWe describe the behavior of the snail Megalobulimus abbreviatus upon receiving thermal stimuli and the effects of pretreatment with morphine and naloxone on behavior after a thermal stimulus, in order to establish a useful model for nociceptive experiments. Snails submitted to non-functional (22ºC) and non-thermal hot-plate stress (30ºC) only displayed exploratory behavior. However, the animals submitted to a thermal stimulus (50ºC) displayed biphasic avoidance behavior. Latency was measured from the time the animal was placed on the hot plate to the time when the animal lifted the head-foot complex 1 cm from the substrate, indicating aversive thermal behavior. Other animals were pretreated with morphine (5, 10, 20 mg/kg) or naloxone (2.5, 5.0, 7.5 mg/kg) 15 min prior to receiving a thermal stimulus (50ºC; N = 9 in each group). The results (means ± SD) showed an extremely significant difference in response latency between the group treated with 20 mg/kg morphine (63.18 ± 14.47 s) and the other experimental groups (P < 0.001). With 2.5 mg/kg (16.26 ± 3.19 s), 5.0 mg/kg (11.53 ± 1.64 s) and 7.5 mg/kg naloxone (7.38 ± 1.6 s), there was a significant, not dose-dependent decrease in latency compared to the control (33.44 ± 8.53 s) and saline groups (29.1 ± 9.91 s). No statistically significant difference was found between the naloxone-treated groups. With naloxone plus morphine, there was a significant decrease in latency when compared to all other groups (minimum 64% in the saline group and maximum 83.2% decrease in the morphine group). These results provide evidence of the involvement of endogenous opioid peptides in the control of thermal withdrawal behavior in this snail, and reveal a stereotyped and reproducible avoidance behavior for this snail species, which could be studied in other pharmacological and neurophysiological studies.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofBrazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas. Ribeirão Preto, SP. Vol. 38, no. 1 (Jan. 2005), p. 73-80pt_BR
dc.rightsOpen Accessen
dc.subjectSnailen
dc.subjectFisiologiapt_BR
dc.subjectThermal avoidanceen
dc.subjectNociceptionen
dc.subjectMorphineen
dc.subjectNaloxoneen
dc.titleThe terrestrial Gastropoda megalobulimus abbreviatus as a useful model for nociceptive experiments : effects of morphine and naloxone on thermal avoidance behaviorpt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb000519397pt_BR
dc.type.originNacionalpt_BR


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