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dc.contributor.authorBurin, Maira Graeffpt_BR
dc.contributor.authorDutra Filho, Carlos Severopt_BR
dc.contributor.authorBrum, J.pt_BR
dc.contributor.authorMauricio, T.pt_BR
dc.contributor.authorAmorim, M.pt_BR
dc.contributor.authorGiugliani, Robertopt_BR
dc.date.accessioned2010-04-24T04:15:32Zpt_BR
dc.date.issued2000pt_BR
dc.identifier.issn0100-879Xpt_BR
dc.identifier.urihttp://hdl.handle.net/10183/21161pt_BR
dc.description.abstractThis study was designed to evaluate the effect of different conditions of collection, transport and storage on the quality of blood samples from normal individuals in terms of the activity of the enzymes ß- glucuronidase, total hexosaminidase, hexosaminidase A, arylsulfatase A and ß-galactosidase. The enzyme activities were not affected by the different materials used for collection (plastic syringes or vacuum glass tubes). In the evaluation of different heparin concentrations (10% heparin, 5% heparin, and heparinized syringe) in the syringes, it was observed that higher doses resulted in an increase of at least 1-fold in the activities of ß-galactosidase, total hexosaminidase and hexosaminidase A in leukocytes, and ß-glucuronidase in plasma. When the effects of time and means of transportation were studied, samples that had been kept at room temperature showed higher deterioration with time (72 and 96 h) before processing, and in this case it was impossible to isolate leukocytes from most samples. Comparison of heparin and acid citrate-dextrose (ACD) as anticoagulants revealed that ß-glucuronidase and hexosaminidase activities in plasma reached levels near the lower normal limits when ACD was used. In conclusion, we observed that heparin should be used as the preferable anticoagulant when measuring these lysosomal enzyme activities, and we recommend that, when transport time is more than 24 h, samples should be shipped by air in a styrofoam box containing wet ice.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofBrazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas. Ribeirão Preto, SP. Vol. 33, no. 9 (Sept. 2000), p. 1003-1013pt_BR
dc.rightsOpen Accessen
dc.subjectLysosomal storage diseasesen
dc.subjectBioquímicapt_BR
dc.subjectLysosomal enzymesen
dc.subjectInborn errors of metabolismen
dc.subjectReference laboratoriesen
dc.subjectStorage of blood samplesen
dc.subjectSample handlingen
dc.titleEffect of collection, transport, processing and storage of blood specimens on the activity of lysomal enzimes in plasma and leukocytespt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb000297152pt_BR
dc.type.originNacionalpt_BR


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