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dc.contributor.authorKim, Tae Kwonpt_BR
dc.contributor.authorTirloni, Lucaspt_BR
dc.contributor.authorPinto, Antonio Frederico Michelpt_BR
dc.contributor.authorDiedrich, Jolene K.pt_BR
dc.contributor.authorMoresco, Jamespt_BR
dc.contributor.authorYates III, John R.pt_BR
dc.contributor.authorVaz Junior, Itabajara da Silvapt_BR
dc.contributor.authorMulenga, Albertpt_BR
dc.date.accessioned2020-03-25T04:16:25Zpt_BR
dc.date.issued2020pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/206907pt_BR
dc.description.abstractAmblyomma americanum ticks transmit more than a third of human tick-borne disease (TBD) agents in the United States. Tick saliva proteins are critical to success of ticks as vectors of TBD agents, and thus might serve as targets in tick antigen-based vaccines to prevent TBD infections. We describe a systems biology approach to identify, by LC-MS/MS, saliva proteins (tick = 1182, rabbit = 335) that A. americanum ticks likely inject into the host every 24 h during the first 8 days of feeding, and towards the end of feeding. Searching against entries in GenBank grouped tick and rabbit proteins into 27 and 25 functional categories. Aside from housekeeping-like proteins, majority of tick saliva proteins belong to the tick-specific (no homology to non-tick organisms: 32%), protease inhibitors (13%), proteases (8%), glycine-rich proteins (6%) and lipocalins (4%) categories. Global secretion dynamics analysis suggests that majority (74%) of proteins in this study are associated with regulating initial tick feeding functions and transmission of pathogens as they are secreted within 24–48 h of tick attachment. Comparative analysis of the A. americanum tick saliva proteome to five other tick saliva proteomes identified 284 conserved tick saliva proteins: we speculate that these regulate critical tick feeding functions and might serve as tick vaccine antigens. We discuss our findings in the context of understanding A. americanum tick feeding physiology as a means through which we can find effective targets for a vaccine against tick feeding.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofPLOS Neglected Tropical Diseases. San Francisco, CA. Vol. 14, no. 2 (Feb. 2020), e0007758, 32 p.pt_BR
dc.rightsOpen Accessen
dc.subjectAmblyomma americanumpt_BR
dc.subjectProteínas e peptídeos salivarespt_BR
dc.subjectAlimentação animalpt_BR
dc.subjectProteômicapt_BR
dc.titleTime-resolved proteomic profile of Amblyomma americanum tick saliva during feedingpt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb001113688pt_BR
dc.type.originEstrangeiropt_BR


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