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dc.contributor.authorCosta, Flávia Póvoa dapt_BR
dc.contributor.authorGomes, Bruna Puty Silvapt_BR
dc.contributor.authorNogueira, Lygia Segapt_BR
dc.contributor.authorMitre, Geovanni Pereirapt_BR
dc.contributor.authorSantos, Sávio Monteiro dospt_BR
dc.contributor.authorTeixeira, Bruno José Britopt_BR
dc.contributor.authorKataoka, Maria Sueli da Silvapt_BR
dc.contributor.authorMartins, Manoela Dominguespt_BR
dc.contributor.authorBarboza, Carlos Augusto Galvãopt_BR
dc.contributor.authorMonteiro, Marta Chagaspt_BR
dc.contributor.authorRogez, Herve Louis Ghislainpt_BR
dc.contributor.authorOliveira, Edivaldo Herculano Corrêapt_BR
dc.contributor.authorLima, Rafael Rodriguespt_BR
dc.date.accessioned2020-03-04T04:17:31Zpt_BR
dc.date.issued2020pt_BR
dc.identifier.issn2076-3921pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/206376pt_BR
dc.description.abstractPiceatannol is a resveratrol metabolite that is considered a potent antioxidant and cytoprotector because of its high capacity to chelate/sequester reactive oxygen species. In pathogenesis of periodontal diseases, the imbalance of reactive oxygen species is closely related to the disorder in the cells and may cause changes in cellular metabolism and mitochondrial activity, which is implicated in oxidative stress status or even in cell death. In this way, this study aimed to evaluate piceatannol as cytoprotector in culture of human periodontal ligament fibroblasts through in vitro analyses of cell viability and oxidative stress parameters after oxidative stress induced as an injury simulator. Fibroblasts were seeded and divided into the following study groups: control, vehicle, control piceatannol, H2O2 exposure, and H2O2 exposure combined with the maintenance in piceatannol ranging from 0.1 to 20 µM. The parameters analyzed following exposure were cell viability by trypan blue exclusion test, general metabolism status by the 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) method, mitochondrial activity through the ATP production, total antioxidant capacity, and reduced gluthatione. Piceatannol was shown to be cytoprotective due the maintenance of cell viability between 1 and 10 µM even in the presence of H2O2. In a concentration of 0.1 µM piceatannol decreased significantly cell viability but increased cellular metabolism and antioxidant capacity of the fibroblasts. On the other hand, the fibroblasts treated with piceatannol at 1 µM presented low metabolism and antioxidant capacity. However, piceatannol did not protect cells from mitochondrial damage as measured by ATP production. In summary, piceatannol is a potent antioxidant in low concentrations with cytoprotective capacity, but it does not prevent all damage caused by hydrogen peroxide.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofAntioxidants. Basel. Vol. 9, no. 1 (Jan. 2020), p. 1-14, artigo 16pt_BR
dc.rightsOpen Accessen
dc.subjectEstilbenospt_BR
dc.subjectStilbenesen
dc.subjectHydrogen Peroxideen
dc.subjectPeróxido de hidrogêniopt_BR
dc.subjectPeriodontal Ligamenten
dc.subjectLigamento periodontalpt_BR
dc.subjectOxidative Stressen
dc.subjectEstresse oxidativopt_BR
dc.subjectViabilityen
dc.subjectpiceatannolen
dc.titlePiceatannol Increases Antioxidant Defense and Reduces Cell Death in Human Periodontal Ligament Fibroblast under Oxidative Stresspt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb001110460pt_BR
dc.type.originEstrangeiropt_BR


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