Mostrar el registro sencillo del ítem

dc.contributor.authorBobrowski, Vera Lúciapt_BR
dc.contributor.authorPasquali, Giancarlopt_BR
dc.contributor.authorBodanese-Zanettini, Maria Helenapt_BR
dc.contributor.authorFiuza, Lidia Marianapt_BR
dc.date.accessioned2010-04-16T09:11:02Zpt_BR
dc.date.issued2001pt_BR
dc.identifier.issn1517-8382pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/19642pt_BR
dc.description.abstractThe bacterium Bacillus thuringiensis (Bt) is characterized by its ability to produce proteic crystalline inclusions during sporulation. Cry1 protein has insecticidal activity and is highly specific to certain insects and not toxic to unrelated insects, plants or vertebrates. In this work, the patogenicity of twelve Bt isolates was tested against Anticarsia gemmatalis, one of the most important insect pests of soybeans. Spore-crystal complex was applied to the surface of artificial diets and the mortality of A. gemmatalis larvae was assessed seven days after each treatment. When compared to a control Bt isolate known by its high toxicity to A. gemmatalis larvae, four novel Bt isolates exhibited even higher toxic activities against the insect, resulting in more than 90% mortality. PCR was used to amplify DNA fragments related to known cry1 genes. Bt strains with high toxicity produced expected PCR products of around 280 bp, whereas non-toxic or low toxic strains did not produce any PCR product or showed amplified fragments of different sizes. Toxic Bt isolates also exhibited an expected protein profile when total protein extracts were evaluated by SDS-PAGE.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofBrazilian journal of microbiology. São Paulo, SP. Vol. 32, n. 2 (abr./jun. 2001), p. 105-109pt_BR
dc.rightsOpen Accessen
dc.subjectBacillus thuringiensisen
dc.subjectBacillus thuringiensispt_BR
dc.subjectVelvetbean caterpillaren
dc.subjectAnticarsia gemmatalispt_BR
dc.subjectGenept_BR
dc.subjectAnticarsia gemmatalisen
dc.subjectSojapt_BR
dc.subjectCry1 geneen
dc.subjectInseticidapt_BR
dc.subjectToxicidadept_BR
dc.subjectProteínapt_BR
dc.titleDetection of cry1 genes in Bacillus thuringiensis isolates from South of Brazil and activity against Anticarsia gemmatalis (Lepidoptera: Noctuidae)pt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb000366659pt_BR
dc.type.originNacionalpt_BR


Ficheros en el ítem

Thumbnail
   

Este ítem está licenciado en la Creative Commons License

Mostrar el registro sencillo del ítem