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dc.contributor.authorMeneghetti, Karine Lenapt_BR
dc.contributor.authorCanabarro, Micaela do Cantopt_BR
dc.contributor.authorOtton, Letícia Munerpt_BR
dc.contributor.authorHain, Thaís dos Santospt_BR
dc.contributor.authorGeimba, Mercedes Passospt_BR
dc.contributor.authorCorção, Gertrudespt_BR
dc.date.accessioned2018-12-22T04:07:08Zpt_BR
dc.date.issued2018pt_BR
dc.identifier.issn1471-2091pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/187344pt_BR
dc.description.abstractBacterial contamination remains the major problem in skin banks, even after antimicrobial treatment, and results in high rates of tissue discarding. This study aimed to analyze bacterial contamination in 32 human skin allografts from the skin bank of Dr. Roberto Corrêa Chem from the Hospital Complex Santa Casa de Misericórdia de Porto Alegre. These samples were already discarded due to microbial contamination. The identification of the bacteria isolated from skin allografts was performed by matrix assisted laser desorption ionization–time of flight. The antimicrobial susceptibility of the isolates to six different classes of antimicrobials was determined using the diskdiffusion agar method, and the evaluation of the inhibitory potential was determined by the minimal inhibitory concentration (50/90) of antimicrobials already used in the skin bank and those that most isolates were susceptible to. A total of 21 (65.6%) skin samples were contaminated with Gram-positive bacteria: 1 (4.7%) with Paenibacillus sp., 12 (61.9%) with Bacillus sp., 6 (28.5%) with Staphylococcus sp., and 2 (9.5%) with Bacillus sp. and Staphylococcus sp. Several resistance profiles, including multiresistance, were found among the isolates. Most of the isolates were susceptible to at least one of the antimicrobials used in the skin bank. All isolates were susceptible to amikacin, gentamicin, and tetracycline, which demonstrated the best inhibitory activities against the isolates and were considered as potential candidates for new antimicrobial treatments. Bacillus, Paenibacillus, and Staphylococcus were isolated from the skin allografts, thus demonstrating the predominance of Gram-positive bacteria contamination. Other factors not related to the resistance phenotype may also be involved in the persistence of bacterial isolates in the skin allografts after antibiotic treatment. Gentamicin, amikacin, and tetracycline can be considered as an option for a more effective treatment cocktail.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofBMC Microbiology. London. Vol. 18, no. 121 (Sep. 2018), p. 1-9pt_BR
dc.rightsOpen Accessen
dc.subjectCurativos biológicospt_BR
dc.subjectSkin allograften
dc.subjectBacterial contaminationen
dc.subjectAloenxertospt_BR
dc.subjectAntimicrobial resistanceen
dc.subjectResistência microbiana a medicamentospt_BR
dc.subjectSkin banken
dc.subjectBancos de tecidospt_BR
dc.subjectDiscardsen
dc.titleBacterial contamination of human skin allografts and antimicrobial resistance : a skin bank problempt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb001082504pt_BR
dc.type.originEstrangeiropt_BR


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