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dc.contributor.authorValdez, Remberto Marcelo Argandoñapt_BR
dc.contributor.authorDuque, Cristianept_BR
dc.contributor.authorCaiaffa, K. S.pt_BR
dc.contributor.authorSantos, Vanessa Rodriguespt_BR
dc.contributor.authorLoesch, Maria Luiza de Aguiarpt_BR
dc.contributor.authorColombo, Natália Helenapt_BR
dc.contributor.authorArthur, Rodrigo Alexpt_BR
dc.contributor.authorNegrini, Thais de Cássiapt_BR
dc.contributor.authorBoriollo, Marcelo Fabianopt_BR
dc.contributor.authorDelbem, Alberto Carlos Botazzopt_BR
dc.date.accessioned2018-10-30T02:38:12Zpt_BR
dc.date.issued2017pt_BR
dc.identifier.issn1472-6831pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/184158pt_BR
dc.description.abstractBackground: Early childhood caries (ECC) is an aggressive condition that can affect teeth of young children. This study aimed to evaluate genotypic diversity and phenotypic traits of S. mutans isolated from dental biofilms of children with different caries status in comparison with caries free (CF) children. Methods: Streptococcus mutans strains were isolated from supragingival biofilm samples of CF, ECC and severe-ECC (S-ECC) children and genotyped by arbitrary-primer polymerase chain reaction - AP-PCR. S. mutans genotypes were tested for their ability to reduce the suspension pH through glycolysis, to tolerate extreme acid challenge and by their ability to form biofilm. Response variables were analyzed by ANOVA/Tukey or Kruskal-Wallis/Mann-Whitney tests at a 5% of significance. Results: There was an increase in the prevalence of Streptococcus mutans in biofilms with the severity of dental caries. No differences in genotypic diversity and in acidogenicity of genotypes were found among CF, ECC and S-ECC children. S mutans strains with genotypes more characteristic for ECC and S-ECC children formed more biofilms than those identified in CF children. The strains isolated from S-ECC children were highly acid tolerant. Conclusion: Although S. mutans genotypic diversity was similar among the groups of children, phenotypic traits of S. mutans, especially the acid tolerance response, could explain the severity of early childhood caries.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofBMC oral health. London. Vol. 17 (2017), p. 1-9, article 115pt_BR
dc.rightsOpen Accessen
dc.subjectCárie dentáriapt_BR
dc.subjectEarly childhood cariesen
dc.subjectStreptococcus mutansen
dc.subjectCriançapt_BR
dc.subjectDental biofilmen
dc.subjectStreptococcus mutanspt_BR
dc.subjectGenotypic diversityen
dc.subjectBiofilmespt_BR
dc.subjectAcidogenicityen
dc.subjectAciduricityen
dc.titleGenotypic diversity and phenotypic traits of Streptococcus mutans isolates and their relation to severity of early childhood cariespt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb001052472pt_BR
dc.type.originEstrangeiropt_BR


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