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dc.contributor.authorBortolon, Liane Balvedi Poerschpt_BR
dc.contributor.authorPereira, Jorge Fernandopt_BR
dc.contributor.authorNhani Junior, Antoniopt_BR
dc.contributor.authorGonzáles, Hebert Hernán Sotopt_BR
dc.contributor.authorTorres, Gisele Abigail Montanpt_BR
dc.contributor.authorConsoli, Lucianopt_BR
dc.contributor.authorArenhart, Rafael Augustopt_BR
dc.contributor.authorBodanese-Zanettini, Maria Helenapt_BR
dc.contributor.authorMargis-Pinheiro, Márciapt_BR
dc.date.accessioned2017-12-05T02:22:00Zpt_BR
dc.date.issued2016pt_BR
dc.identifier.issn1415-4757pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/170810pt_BR
dc.description.abstractDrought limits wheat production in the Brazilian Cerrado biome. In order to search for candidate genes associated to the response to water deficit, we analyzed the gene expression profiles, under severe drought stress, in roots and leaves of the cultivar MGS1 Aliança, a well-adapted cultivar to the Cerrado. A set of 4,422 candidate genes was found in roots and leaves. The number of down-regulated transcripts in roots was higher than the up-regulated transcripts, while the opposite occurred in leaves. The number of common transcripts between the two tissues was 1,249, while 2,124 were specific to roots and 1,049 specific to leaves. Quantitative RT-PCR analysis revealed a 0.78 correlation with the expression data. The candidate genes were distributed across all chromosomes and component genomes, but a greater number was mapped on the B genome, particularly on chromosomes 3B, 5B and 2B. When considering both tissues, 116 different pathways were induced. One common pathway, among the top three activated pathways in both tissues, was starch and sucrose metabolism. These results pave the way for future marker development and selection of important genes and are useful for understanding the metabolic pathways involved in wheat drought response.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofGenetics and molecular biology. Ribeirão Preto, SP. Vol. 39, no. 4 (Dec. 2016), p. 629-645pt_BR
dc.rightsOpen Accessen
dc.subject454 sequencingen
dc.subjectTriticum aestivumpt_BR
dc.subjectCandidate genesen
dc.subjectDeficits hidricospt_BR
dc.subjectReação em cadeia da polimerase em tempo realpt_BR
dc.subjectRT-qPCRen
dc.subjectTriticum aestivumen
dc.subjectWater deficiten
dc.titleGene expression analysis reveals important pathways for drought response in leaves and roots of a wheat cultivar adapted to rainfed cropping in the Cerrado Biomept_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb001026956pt_BR
dc.type.originNacionalpt_BR


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