Otimização e validação de método para análise simultânea de solventes orgânicos em fluido oral coletado por quantisal tm
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Date
2013Author
Advisor
Co-advisor
Academic level
Graduation
Title alternative
Optimization and validation of a method for simultaneous analysis of organic solvents in oral fluid collected by Quantisal™
Subject
Abstract
The development of analytical methods is required for the biological monitoring of occupational exposure. Considering that oral fluid (OF) is a promising matrix, the aim of this study was to optimize the headspace extraction by using the central composite design and to validate a methodology for the simultaneous analysis of ethyl ether, dichloromethane and ethyl acetate in OF by headspace-gas chromatography with mass spectrometry detector. The best condition chosen for the headspace analysis wa ...
The development of analytical methods is required for the biological monitoring of occupational exposure. Considering that oral fluid (OF) is a promising matrix, the aim of this study was to optimize the headspace extraction by using the central composite design and to validate a methodology for the simultaneous analysis of ethyl ether, dichloromethane and ethyl acetate in OF by headspace-gas chromatography with mass spectrometry detector. The best condition chosen for the headspace analysis was 85°C for 5 minutes. The method was satisfactorily validated, making it adequate for use in toxicological analysis of occupational exposure to organic solvents. ...
Institution
Universidade Federal do Rio Grande do Sul. Faculdade de Farmácia. Curso de Farmácia.
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