QMix® irrigant reduces lipopolysacharide (LPS) levels in an in vitro model
Fecha
2015Autor
Materia
Abstract
The presence of endotoxin inside the root canal has been associated with periapical in vitro, the effect of QMix® and other three root canal irrigants in reducing the endotoxin content in root canals. Material and Methods: Root canals of single-rooted teeth were prepared. Samples were detoxified with Co-60 irradiations and inoculates with E. coli LPS (24 h, at 37°C). After that period, samples were divided into 4 groups, according to the irrigation solution tested: QMix®, 17% EDTA, 2% chlorhexi ...
The presence of endotoxin inside the root canal has been associated with periapical in vitro, the effect of QMix® and other three root canal irrigants in reducing the endotoxin content in root canals. Material and Methods: Root canals of single-rooted teeth were prepared. Samples were detoxified with Co-60 irradiations and inoculates with E. coli LPS (24 h, at 37°C). After that period, samples were divided into 4 groups, according to the irrigation solution tested: QMix®, 17% EDTA, 2% chlorhexidine solution (CHX), and 3% sodium hypochlorite (NaOCl). LPS quantification was determined by Limulus Amebocyte Lysate (LAL) assay. The initial counting of endotoxins for all samples, and the determination of LPS levels in non-contaminated teeth and in contaminated teeth exposed only to nonpyrogenic water, were used as controls. Results: QMix® reduced LPS levels, with a median value of 1.11 endoxins units (EU)/mL (p<0.001). NaOCl (25.50 EU/mL), chlorhexidine (44.10 EU/mL) and positive control group (26.80 EU/mL) samples had similar results. Higher levels were found with EDTA (176.00 EU/mL) when compared to positive control (p<0.001). There was no significant difference among EDTA, NaOCl and CHX groups. Negative control group (0.005 EU/mL) had statistically significant lower levels of endotoxins when compared to al test groups (p<0.001). Conclusion: QMix® decreased LPS levels when compared to the other groups (p<0.001). 3% NaOCl, 2% CHX and 17% EDTA were not able to significantly reduce the root canal endotoxins load ...
En
Journal of applied oral science. Bauru. Vol. 23, no. 4 (July/Aug. 2015), p. 431-435
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