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dc.contributor.authorGalli, Vanessapt_BR
dc.contributor.authorGuzman, Frankpt_BR
dc.contributor.authorOliveira, Luiz Felipe Valter dept_BR
dc.contributor.authorMorais, Guilherme Loss dept_BR
dc.contributor.authorKorbes, Ana Paulapt_BR
dc.contributor.authorSilva, Sérgio Delmar dos Anjos ept_BR
dc.contributor.authorMargis-Pinheiro, Márciapt_BR
dc.contributor.authorMargis, Rogeriopt_BR
dc.date.accessioned2015-04-14T01:57:42Zpt_BR
dc.date.issued2014pt_BR
dc.identifier.issn1932-6203pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/115268pt_BR
dc.description.abstractMicroRNAs, or miRNAs, are endogenously encoded small RNAs that play a key role in diverse plant biological processes. Jatropha curcas L. has received significant attention as a potential oilseed crop for the production of renewable oil. Here, a sRNA library of mature seeds and three mRNA libraries from three different seed development stages were generated by deep sequencing to identify and characterize the miRNAs and pre-miRNAs of J. curcas. Computational analysis was used for the identification of 180 conserved miRNAs and 41 precursors (pre-miRNAs) as well as 16 novel pre-miRNAs. The predicted miRNA target genes are involved in a broad range of physiological functions, including cellular structure, nuclear function, translation, transport, hormone synthesis, defense, and lipid metabolism. Some pre-miRNA and miRNA targets vary in abundance between the three stages of seed development. A search for sequences that produce siRNA was performed, and the results indicated that J. curcas siRNAs play a role in nuclear functions, transport, catalytic processes and disease resistance. This study presents the first large scale identification of J. curcas miRNAs and their targets in mature seeds based on deep sequencing, and it contributes to a functional understanding of these miRNAs.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofPLoS ONE. San Francisco. Vol. 9, no. 2 (Feb. 2014), e83727, 10 p.pt_BR
dc.rightsOpen Accessen
dc.subjectMicroRNAspt_BR
dc.subjectJartropha seedspt_BR
dc.titleIdentifying MicroRNAs and transcript targets in Jartropha seedspt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb000920358pt_BR
dc.type.originEstrangeiropt_BR


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