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dc.contributor.authorStaats, Charley Christianpt_BR
dc.contributor.authorJunges, Angelapt_BR
dc.contributor.authorGuedes, Rafael Lucas Munizpt_BR
dc.contributor.authorThompson, Claudia Elizabethpt_BR
dc.contributor.authorMorais, Guilherme Loss dept_BR
dc.contributor.authorBoldo, Juliano Tomazzonipt_BR
dc.contributor.authorAlmeida, Luiz Gonzaga Paula dept_BR
dc.contributor.authorAndreis, Fábio Carrerpt_BR
dc.contributor.authorGerber, Alexandra Lehmkuhlpt_BR
dc.contributor.authorOliveira, Nicolau Sbarainipt_BR
dc.contributor.authorPaixão, Rana Louise de Andrade dapt_BR
dc.contributor.authorBroetto, Leonardopt_BR
dc.contributor.authorLandell, Melissa Fontespt_BR
dc.contributor.authorSanti, Lucéliapt_BR
dc.contributor.authorSilva, Walter Orlando Beys dapt_BR
dc.contributor.authorSilveira, Carolina Pereirapt_BR
dc.contributor.authorSerrano, Thaiane Rispolipt_BR
dc.contributor.authorOliveira, Eder Silva dept_BR
dc.contributor.authorSilva, Lívia Kmetzsch Rosa ept_BR
dc.contributor.authorVainstein, Marilene Henningpt_BR
dc.contributor.authorVasconcelos, Ana Tereza Ribeiro dept_BR
dc.contributor.authorSchrank, Augustopt_BR
dc.date.accessioned2015-04-08T01:58:47Zpt_BR
dc.date.issued2014pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/115041pt_BR
dc.description.abstractBackground: Metarhizium anisopliae is an entomopathogenic fungus used in the biological control of some agricultural insect pests, and efforts are underway to use this fungus in the control of insect-borne human diseases. A large repertoire of proteins must be secreted by M. anisopliae to cope with the various available nutrients as this fungus switches through different lifestyles, i.e., from a saprophytic, to an infectious, to a plant endophytic stage. To further evaluate the predicted secretome of M. anisopliae, we employed genomic and transcriptomic analyses, coupled with phylogenomic analysis, focusing on the identification and characterization of secreted proteins. Results: We determined the M. anisopliae E6 genome sequence and compared this sequence to other entomopathogenic fungi genomes. A robust pipeline was generated to evaluate the predicted secretomes of M. anisopliae and 15 other filamentous fungi, leading to the identification of a core of secreted proteins. Transcriptomic analysis using the tick Rhipicephalus microplus cuticle as an infection model during two periods of infection (48 and 144 h) allowed the identification of several differentially expressed genes. This analysis concluded that a large proportion of the predicted secretome coding genes contained altered transcript levels in the conditions analyzed in this study. In addition, some specific secreted proteins from Metarhizium have an evolutionary history similar to orthologs found in Beauveria/Cordyceps. This similarity suggests that a set of secreted proteins has evolved to participate in entomopathogenicity. Conclusions: The data presented represents an important step to the characterization of the role of secreted proteins in the virulence and pathogenicity of M. anisopliae.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofBMC Genomics. London. Vol. 15, (Sept. 2014), e822, 18 p.pt_BR
dc.rightsOpen Accessen
dc.subjectFungo entomopatogenicopt_BR
dc.subjectGenome sequenceen
dc.subjectEntomopathogenic fungien
dc.subjectGenomapt_BR
dc.subjectSecretomeen
dc.subjectPhylogenomicsen
dc.titleComparative genome analysis of entomopathogenic fungi reveals a complex set of secreted proteinspt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb000955042pt_BR
dc.type.originEstrangeiropt_BR


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