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dc.contributor.authorGonçalves, Juliana Wolmannpt_BR
dc.contributor.authorValiati, Victor Hugopt_BR
dc.contributor.authorDelprat, Alejandrapt_BR
dc.contributor.authorGaiesky, Vera Lúcia da Silva Valentept_BR
dc.contributor.authorRuiz, Alfredopt_BR
dc.date.accessioned2015-04-08T01:58:47Zpt_BR
dc.date.issued2014pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/115040pt_BR
dc.description.abstractBackground: Galileo is one of three members of the P superfamily of DNA transposons. It was originally discovered in Drosophila buzzatii, in which three segregating chromosomal inversions were shown to have been generated by ectopic recombination between Galileo copies. Subsequently, Galileo was identified in six of 12 sequenced Drosophila genomes, indicating its widespread distribution within this genus. Galileo is strikingly abundant in Drosophila willistoni, a neotropical species that is highly polymorphic for chromosomal inversions, suggesting a role for this transposon in the evolution of its genome. Results: We carried out a detailed characterization of all Galileo copies present in the D. willistoni genome. A total of 191 copies, including 133 with two terminal inverted repeats (TIRs), were classified according to structure in six groups. The TIRs exhibited remarkable variation in their length and structure compared to the most complete copy. Three copies showed extended TIRs due to internal tandem repeats, the insertion of other transposable elements (TEs), or the incorporation of non-TIR sequences into the TIRs. Phylogenetic analyses of the transposase (TPase)-encoding and TIR segments yielded two divergent clades, which we termed Galileo subfamilies V and W. Target-site duplications (TSDs) in D. willistoni Galileo copies were 7- or 8-bp in length, with the consensus sequence GTATTAC. Analysis of the region around the TSDs revealed a target site motif (TSM) with a 15-bp palindrome that may give rise to a stem-loop secondary structure. Conclusions: There is a remarkable abundance and diversity of Galileo copies in the D. willistoni genome, although no functional copies were found. The TIRs in particular have a dynamic structure and extend in different ways, but their ends (required for transposition) are more conserved than the rest of the element. The D. willistoni genome harbors two Galileo subfamilies (V and W) that diverged ~9 million years ago and may have descended from an ancestral element in the genome. Galileo shows a significant insertion preference for a 15-bp palindromic TSM.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofBMC Genomics. London. Vol. 15, (Sept. 2013), e792, 11 p.pt_BR
dc.rightsOpen Accessen
dc.subjectTransposable elementen
dc.subjectDrosophila willistonipt_BR
dc.subjectGenomapt_BR
dc.subjectD. willistonien
dc.subjectTerminal inverted repeatsen
dc.subjectP superfamilyen
dc.subjectTarget site duplicationsen
dc.titleStructural and sequence diversity of the transposon Galileo in the Drosophila willistoni genomept_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb000955033pt_BR
dc.type.originEstrangeiropt_BR


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