LC-MS/MS method applied to preclinical pharmacokinetic investigation of olanzapine-loaded lipid-core nanocapsules
Fecha
2014Autor
Materia
Abstract
In spite of different methods reported in the literature to determine olanzapine in biological fluids, all of them used high volumes of plasma. Therefore, the purpose of this paper was to develop an LC-MS/MS method using small plasma volume (0.1 mL) to apply in a preclinical pharmacokinetic investigation. The method was linear over the concentration ranges of 10–1000 ng mL−1. Extraction recoveries, stability, and validation parameters were evaluated. Results were within the acceptable limits of ...
In spite of different methods reported in the literature to determine olanzapine in biological fluids, all of them used high volumes of plasma. Therefore, the purpose of this paper was to develop an LC-MS/MS method using small plasma volume (0.1 mL) to apply in a preclinical pharmacokinetic investigation. The method was linear over the concentration ranges of 10–1000 ng mL−1. Extraction recoveries, stability, and validation parameters were evaluated. Results were within the acceptable limits of international guidelines. A significant decrease in clearance led to a significant 2.26-times increase in AUC0–6h of olanzapine-loaded lipid-core nanocapsules compared with free-olanzapine. ...
En
Química nova. São Paulo. Vol. 37, n. 8 (2014), p. 1371-1376
Origen
Nacional
Colecciones
-
Artículos de Periódicos (39042)Ciencias Exactas y Naturales (5930)
-
Artículos de Periódicos (39042)Ciencias de la Salud (10539)
Este ítem está licenciado en la Creative Commons License